Application

This Anti-Derlin-1 Antibody, clone 8G11.2 is validated for use in Western Blotting, Immunocytochemistry for the detection of Derlin-1.

Research CategorySignaling

Immunocytochemistry Analysis: An 1:50 dilution from a representative lot detected Derlin-1 in HUVECs, A431 and HeLa cells.

Research Sub CategoryUbiquitin & Ubiquitin Metabolism

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Derlin-1 (UniProt Q9BUN8; also known as Degradation in endoplasmic reticulum protein 1, Der1-like protein 1, DERtrin-1) is encoded by the DERL1 (also known as DER1) gene (ORF UNQ243/PRO276; Gene ID 79139) in human. Derlins are integral ER membrane proteins that form homo-oligomers and heteromultimeric complexes with membrane-bound machinery, including the E3 ubiquitin ligase Hrd1 and its binding partner SEL1L, involved in ER-associated degradation (ERAD). Before ERAD substrates can be processed by the cytoplasmic ubiquitin-proteasome system, they need to be translocated across the ER membrane through an AAA ATPase p97/VCP-driven process. Derlins are inactive members of the rhomboid family of intramembrane proteases that mediate the recruitment of AAA ATPase p97/VCP through interaction via their C-terminal SHP boxes. Derlin-1 is proposed to contain four transmembrane segments (a.a. 23-43, 60-80, 106-126, 155-175), three cytoplasmic domains (a.a. 23-43, 60-80, 106-126, 155-175), and two lumenol (a.a. 44-59 & 127-154) domains.

Immunogen

GST-tagged recombinant human derlin-1 C-terminal cytoplasmic fragment.

Epitope: C-terminal cytoplasmic domain.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Derlin-1 in 10 µg of HEK293 cell lysate.

Specificity

Expected to react with both spliced isoforms of human Derlin-1 reported by UniProt (Q9BUN8).

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~24 kDa observed. 28.80 kDa (isoform 1) and 26.42 kDa (isoform 2) calculated.